Clariostar Plus

CLARIOstar Plus

• Detection Modes

  Fluorescence intensity (incl. FRET)

  Fluorescence polarization

  AlphaScreen^®/AlphaLISA^®/AlphaPlex^TM

  Luminescence (flash and glow) - incl. BRET

  Time-resolved fluorescence

  TR-FRET

  UV/vis absorbance spectra

• Measurement Modes

  Top and bottom reading

  Endpoint and kinetic

  Sequential multi-excitation

  Sequential multi-emission

  Spectral scanning (fluorescence, luminescence, absorbance)

  Ratiometric measurements

  Well scanning

• Microplate Formats

  6- to 1536-well plates, user-definable

  LVis Plate with 16 low volume microspots (2 μL)

• Microplate Carrier

  Robot compatible

• Light Sources

  High energy xenon flash lamp

  Dedicated laser for AlphaScreen^®/AlphaLISA^®/AlphaPlex^TM

• Detectors

  Low-noise photomultiplier tube

  Red-shifted photomultiplier tube

  CCD spectrometer

• Wavelength Selection

  Dual Linear Variable Filter (LVF) Monochromators™

  Linear Variable Dichroic Mirror

  Separates ex & em LVF Monochromators

  Optical filters:

  Ex and em slides hold 4 filters each

  LVF Monochromators + optical filters

  Use one for ex and the other for em

  UV/Vis absorbance spectrometer:

  Full spectra or 8 distinct wavelengths in < 1 sec/well

• Optical Filters

  Excitation and emission slides for 4 filters each

• Optical Path Guides

  Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics

• Z-Adjustment

  Automatic focal height adjustment (0.1 mm resolution)

• Spectral Range

  Filters	FI, FP, TRF: 240 - 740 nm or 240 - 900 nm (red-shifted PMT) LUM: 240 - 740 nm
  LVF Monochromators™	FI: 320 - 740 nm or 320 - 840 nm (red-shifted PMT) LUM: 320 - 740 nm
  Linear Variable Dichroic	340 - 740 nm or 340 - 760 nm (red-shifted PMT)
  Spectrometer	ABS: 220 - 1000 nm

• Sensitivity

  FI filters (top)	< 0.15 pM (< 3 amol/well FITC, 384sv, 20 μL)
  FI filters (bottom)	< 1.0 pM (< 50 amol/well FITC, 384g, 50 μL)
  FI monochromator (top)	< 0.35 pM (< 7 amol/well FITC, 384sv, 20 μL)
  FI monochromator (bottom)	< 3.0 pM (< 150 amol/well FITC, 384g, 50 μL) Dynamic Range: 8 decades in a single measurement
  FP	< 0.5 mP SD at 1 nM FITC (384sv, 20 μL)
  HTRF® (black and white microplates)	Reader Control Kit (Eu) after 18h (384sv, 20 μL) Delta F > 880 % (High Calibrator) Delta F > 30 % (Low Calibrator)
  TRF	< 20 fM europium, 384, 80 μL
  LUM	< 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL) Dynamic range: 8 decades in a single measurement
  AlphaScreen® with laser	< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL)
  ABS with spectrometer	Full spectrum captured in < 1 s / well Selectable spectral resolution: 1, 2, 5, and 10 nm OD range: 0 - 4 OD Accuracy: < 1% at 2 OD Precision: < 0.5% at 1 OD and < 0.8% at 2 OD

• Read Times

  Flying mode (1 flash)	8 s (96), 15 s (384), 28 s (1536)
  10 flashes	19 s (96), 57 s (384), 3 min 4 s (1536)

• Reagent Injection

  Up to 2 built-in reagent injectors
  Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL)
  Variable injection speed up to 420 µL / s
  Reagent back flushing

• Shaking

  Linear, orbital, and double-orbital with user-definable time and speed

• Integrated Barcode Reader

  Up to two integrated barcode readers

• Incubation

  +3 °C above ambient up to 45 °C or 65 °C

  The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.

• Software

  Multi-user Reader Control and MARS Data Analysis Software included
  FDA 21 CFR Part 11 compliant

  Integrated fluorophore library

• Dimensions

  Width: 45 cm, depth: 51 cm, height: 40 cm; weight: 32 kg

• Optional Accessories
• LVis Plate

  Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples.

  Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy

  Dimensions: conforms to SBS standards for microplates.

• Atmospheric Control Unit (ACU)

  Actively regulates O₂ and CO₂ - 0.1-20%

• Stacker

  Magazines for up to 50 plates – continuous loading feature

• THERMOstar

  Microplate incubator and shaker

• Optical Filters

  Excitation and emission filters

• Upgrades

  The CLARIOstar Plus can include all or any combination of features listed below at purchase. Upgrading with additional features is possible at any time. Please contact your local representative for more details or a quote.

• *

  LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
  AlphaScreen^® P-Tyr-100 assay kit, PerkinElmer, #6760620C
  Microplates: white for LUM, AlphaScreen^®, TRF; black for FI, FP; clear for ABS
  96 = 96-well microplates
  384sv = 384-well small volume microplates
  384g = 384-well glass bottom microplates
  1536 = 1536-well microplates