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FLUOstar Omega

Item#: FLUOstar-Omega

The FLUOstar® Omega is a multi-mode microplate reader with six detection modes. It utilizes an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. The FLUOstar Omega is the ideal plate reader for life science applications.

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  • Detection Modes

    Fluorescence intensity - incl. FRET

    AlphaScreen®/AlphaLISA®

    Luminescence (flash and glow) - incl. BRET

    Time-resolved fluorescence

    TR-FRET

    UV/Vis absorbance spectra

  • Measurement Modes

    Top and bottom reading

    Endpoint and kinetic

    Sequential multi excitation

    Sequential multi emission

    Spectral scanning (absorbance)

    Ratiometric measurements

    Well scanning

  • Microplate Formats

    6 to 384-well plates, user-definable
    LVis Plate with 16 low volume microspots (2 μL)

  • Microplate Carrier

    Robot compatible

  • Light Sources

    High energy xenon flash lamp
  • Detectors

    Low noise photomultiplier tube
    CCD spectrometer

  • Wavelength Selection

    Optical filters: ex and em wheels for 8 filters each
    UV/vis absorbance spectrometer: full spectra or 8 distinct wavelengths in < 1 sec / well

  • Optical Filters

    Excitation and emission filter wheels for 8 filters each

  • Optical Path Guides

    Top: liquid-filled light guides
    Bottom: fiber optics

  • Spectral Range
    Filters 240 - 740 nm or 240 - 900 nm for FI, FP, TRF
    240 - 740 nm for LUM
    Spectrometer 220 - 1000 nm for ABS
  • Sensitivity
    FI  < 0.2 fmol/well FITC
    TRF < 30 amol/well europium
    High-End TRF for Omega < 3 amol/well europium
    LUM < 20 amol/well ATP
    AlphaScreen® < 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL)
    ABS
    with spectrometer    		 Full spectrum captured in < 1 s/well
    Selectable spectral resolution: 1, 2, 5, and 10 nm
    OD range: 0 to 4 OD
    Accuracy: < 1% at 2 OD
    Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
  • Read Times

    Flying mode (1 flash)
    9 s (96), 16 s (384)

  • Reagent Injection

    Up to 2 built-in reagent injectors
    Injection at measurement position (6 to 384-well)
    Individual injection volumes for each well (3 to 500 µL)
    Variable injection speed up to 420 µL / s
    Up to four injection events per well
    Reagent back flushing

  • Shaking

    Linear, orbital, and double-orbital with user-definable time and speed

  • Purge Gas Vent

    System to inject an atmosphere or to pull a vacuum into the reader

  • Incubation

    +4 °C above ambient up to 45 °C or 65 °C

    The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.

  • Software

    Multi-user Reader Control and MARS Data Analysis Software included
    FDA 21 CFR Part 11 compliant

  • Dimensions

    Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg

  • Optional Accessories
  • LVis Plate

    Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples.

    Quality control internal standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy

    Dimensions: conforms to SBS standards for microplates.

  • Atmospheric Control Unit (ACU)

    Actively regulates O2 and CO2 - 0.1-20%

  • Stacker

    Magazines for up to 50 plates - continuous loading feature

  • THERMOstar

    Microplate incubator and shaker

  • Optical Filters

    Excitation and emission filters

  • Upgrades

    Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc.

  • *

    LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
    AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
    Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
    96 = 96-well microplates
    384sv = 384-well small volume microplates
    384g = 384-well glass bottom microplates

The FLUOstar Omega is a versatile multi-mode microplate reader with six detection modes: fluorescence intensity (including FRET), luminescence (including BRET), UV/vis absorbance, time-resolved fluorescence, TR-FRET, and AlphaScreen®/AlphaLISA®.

Seven certifications of BMG LABTECH microplate reader: HTRF, DLR, LanthaScreen, Transcreener ADP2 FI, FP, TR-FRET, MycoAlert (from left to right)

Features

  • High-speed absorbance measurements
  • Measurements in up to 384-well plates
  • Top and bottom reading
  • Incubation up to 45 or 65 °C
  • User-defined shaking
  • Well scanning with up to 900 data points per well
  • High-precision injectors with simultaneous reagent injection and detection
  • Low-volume measurements

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