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NEPHELOstar Plus

Item#: NEPHELOstar-Plus

The world’s first and only laser-based microplate nephelometer was developed to meet high-throughput demands for fast compound solubility screens. The NEPHELOstar® Plus detects insoluble particles in liquid samples by measuring forward scattered light and can be used for turbidity measurements, drug solubility screening, protein binding kinetics, microbial growth, and petroleum process control.

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  • Detection Modes

    Nephelometry (light scattering)

  • Measurement Modes

    Endpoint and kinetic

  • Microplate Formats

    Up to 384-well plates

  • Microplate Carrier

    Robot compatible

  • Light Sources

    Self-monitoring laser diode
    Wavelength 635±10 nm
    Stability <0.2% deviation
    Lifetime 20,000 hours
    Output: 1 mW
    Selectable beam width: 1.5 to 3.5 mm
    Selectable intensity 0-100%
    Scattering angle: detects up to 80° full cone angle

  • Detectors

    Side window photodiode detector

  • Wavelength Selection

    Photodiode
    Wavelength 635±10 nm

  • Sensitivity

    Depends on particle size and liquid properties
    Silica detection (particle size 0.5 to 10 µm) 800 nM

    Dynamic Range: 5 decades
    Maximum count rate (2,000,000 Relative Nephelometry Units (RNUs) per second)

  • Read Times

    Depend on assay conditions and liquid surface stability
    Shortest times possible: 16 s (96), 47 s (384)

  • Reagent Injection

    Up to 2 built-in reagent injectors
    Injection at measurement position (6 to 384-well)
    Individual injection volumes for each well (3 to 500 µL)
    Variable injection speed up to 420 µL / s
    Up to four injection events per well
    Reagent back flushing

  • Shaking

    Linear, orbital, and double-orbital with user-definable time and speed

  • Purge Gas Vent

    System to inject an atmosphere or to pull a vacuum into the reader

  • Incubation

    +4°C above ambient up to 45°C or 60°C

    The upper heating plate of the incubation chamber operates at 0.5°C more than the lower plate. This prevents condensation build-up on the lid or sealer.

  • Software

    Multi-user Reader Control and MARS Data Analysis Software included
    FDA 21 CFR Part 11 compliant

  • Dimensions

    Width: 44 cm, depth: 48 cm, height: 32 cm; weight: 25 kg

  • Optional Accessories
  • Stacker

    Magazines for up to 50 plates - continuous loading feature

  • THERMOstar

    Microplate incubator and shaker

  • Upgrades

    Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc.

The NEPHELOstar Plus is a microplate nephelometer with a high-intensity laser diode. It can be up to thirty times more sensitive than traditional transmission optical density readers that measure the reduction in direct light passing through a sample well. Thanks to its speed, flexibility and performance, the NEPHELOstar Plus allows more applications to be adapted to microplate-based laser nephelometry than ever before.

Features

  • Adjustable laser intensity and beam diameter
  • Measurement in up to 384-well microplate format
  • Incubation up to 45°C or 60°C
  • Linear, orbital and double-orbital shaking
  • High-precision injectors with simultaneous reagent injection and detection
  • Microplate Stacker and robot compatible

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