PHCbi LiCellMo Live Cell Metabolic Analyzer

In-line sensors: the key to continuous measurements of glucose and lactate

The glycolytic pathway is one of the main components of cellular energy metabolism. During glycolysis, glucose is metabolized and lactate is produced. Conventional analysis of cell metabolism typically involves estimating glucose and lactate concentrations from data points obtained from periodic sampling. The high-precision in-line sensors of LiCellMo offer these unique advantages:

• Real-time monitoring of glucose and lactate concentrations
• Continuous measurements without sampling
• Preservation of cells for further evaluation after measurement
• Comprehensive, quantifiable data

Measure cells in their usual culture environment

The simplicity of LiCellMo's design makes it suitable for any laboratory space and does not require specialized cell culturing equipment. Common commercial products (culture medium, 24-well plates, calibration liquid, additive reagents) can be used for cell culture. The sensor module and plate adaptor can be attached to standard 24-well plates. Once the plate is placed in the detector pre-installed in the CO₂ incubator, real-time, sample-free measurements can be easily monitored using the touch-panel controller. Optional plate adapters are available for five different commercial 24-well plates.

Evaluate glycolytic changes directly

LiCellMo enables direct evaluation of changes in the glycolytic pathway by measuring glucose uptake and lactate production in the cell culture medium. It visualizes cell metabolism using consumption and production rates based on concentration values, providing valuable metabolic rate data. Monitoring the efficiency of conversion from glucose to lactate makes it possible to evaluate not only glycolysis but also the balance with other cellular metabolic processes, such as the TCA cycle and oxidative phosphorylation in mitochondria.