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PHERAstar FSX

Item#: PHERAstar-FSX
The PHERAstar® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, and dedicated AlphaScreen® and TRF lasers, the PHERAstar FSX will perform your assay with ease and speed, even in 3456-well microplates.
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  • Detection Modes

    Fluorescence intensity - incl. FRET

    Fluorescence polarization

    AlphaScreen®/AlphaLISA®/AlphaPlexTM

    Luminescence (flash and glow) - incl. BRET

    Time-resolved fluorescence

    TR-FRET

    UV/Vis absorbance spectra

  • Measurement Modes

    Top and bottom reading

    Endpoint and kinetic

    Sequential multi-excitation

    Sequential multi-emission

    Simultaneous Dual Emission

    Spectral scanning (absorbance)

    Real-time ratiometric measurements

    Well scanning

  • Microplate Formats

    Up to 3456-well plates, user-definable
    LVis Plate with 16 low volume microspots (2 μL)

  • Microplate Carrier

    Robot compatible

  • Light Sources

    High energy xenon flash lamp

    Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM

    Dedicated laser for TRF and TR-FRET

  • Detectors

    Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes
    CCD spectrometer

  • Wavelength Selection

    Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application)

    For top and bottom reading

    UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec / well

  • Optical Path Guides

    Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics

  • Z-Adjustment

    Automatic focal height adjustment (0.1 mm resolution)

  • Spectral Range
    Filters 230 - 750 nm or 230 - 900 nm for FI, FP
    230 - 750 nm for LUM
    230 - 900 nm for TRF
    Spectrometer 220 - 1000 nm for ABS
  • Sensitivity
    FI
    (top)    		 < 0.15 pM FITC (384sv black, 20 μL)
    < 0.5 pM FITC (1536 black, 8 μL)
    FI
    (bottom)    		 < 1.0 pM FITC (384g black, 50 μL)
    FP < 0.5 mP SD at 1 nM FITC (384sv black, 20 μL)
    < 1.5 mP SD at 1 nM FITC (1536 black, 8 μL)
    TRF
    with laser    		 < 5 fM europium (384, 80 μL)
    < 15 fM europium (1536, 8 μL)
    HTRF®
    (black and white microplates)    		 Reader Control Kit (Eu) after 18h (384sv, 20 μL)
    Delta F > 1100 % (High Calibrator)
    Delta F > 25 % (Low Calibrator)
    LUM < 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
    Dynamic Range: 9 decades
    AlphaScreen®
    with laser    		 < 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)
    ABS
    with spectrometer    		 Full spectrum captured in < 1 s / well
    Selectable spectral resolution: 1, 2, 5, and 10 nm
    OD range: 0 - 4 OD
    Accuracy: < 1% at 2 OD
    Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
  • Read Times
    Flying mode
    (1 flash)    		 14 s (384)
    27 s (1536)
    10 flashes 38 s (384)
    1 min 52 s (1536)
    50 flashes 1 min 29 s (384)
    5 min 18 s (1536)
  • Reagent Injection

    Up to 2 built-in reagent injectors

    Injection at measurement position (6 to 384-well)

    Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)

    Variable injection speed up to 420 µL / s

    Up to four injection events per well

    Reagent back flushing

  • Shaking

    Linear, orbital, and double-orbital with user-definable time and speed

  • Integrated Barcode Reader

    Up to three integrated barcode readers

  • Incubation

    +5 °C above ambient up to 45 °C

    The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.

  • Software

    Multi-user Reader Control and MARS Data Analysis Software included
    FDA 21 CFR Part 11 compliant

  • Dimensions

    Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg
  • Optional Accessories
  • LVis Plate

    Sample Capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples.

    Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy.

    Dimensions: conforms to SBS standards for microplates.

  • Stacker

    Magazines for up to 50 plates - continuous loading feature

  • THERMOstar

    Microplate incubator and shaker

  • Upgrades

    Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc.

  • *

    LOD = 3 x SD (20 blanks) / slope (6 pt std curve)

    AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C

    384sv = 384-well small volume microplates

    384g = 384-well glass bottom microplates

    1536 = 1536-well microplates

No longer worry about installing filters and dichroics: the PHERAstar FSX comes with easy-to-handle, assay-optimized Optic Modules which contain all application-specific filters, mirrors, dichroics and/or polarizers, and are automatically recognized by the plate reader. In addition, the PHERAstar FSX is equipped with four matched and assay-optimized photomultiplier tubes (PMTs) which are automatically selected for the relevant detection mode. You can also measure assays with two emission wavelengths at the same time thanks to the Simultaneous Dual Emission.

 

For HTS automation purposes, the PHERAstar FSX offers improved robotic integration capabilities and multi-user control.

Seven certifications of BMG LABTECH microplate reader: HTRF, DLR, LanthaScreen, Transcreener ADP2 FI, FP, TR-FRET, MycoAlert (from left to right)

Features

  • The most sensitive reader in fluorescence intensity and polarization
  • The only plate reader with 5 assay-dedicated detectors
  • Simultaneous Dual Emission, including for Alpha Technology
  • 9 decades luminescence dynamic range
  • Dedicated AlphaScreen®/AlphaLISA®/AlphaPlex™ laser
  • New generation TRF-laser
  • Top and bottom focal height adjustment
  • High-precision injectors with simultaneous reagent injection and detection
  • High-speed absorbance measurements

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