POLARstar Omega

Fluorescence intensity - incl. FRET

Fluorescence polarization

AlphaScreen®/AlphaLISA®

Luminescence (flash and glow) - incl. BRET

Time-resolved fluorescence

TR-FRET

UV/Vis absorbance spectra

Top and bottom reading

Endpoint and kinetic

Sequential multi excitation

Sequential multi emission

Simultaneous Dual Emission

Spectral scanning (absorbance)

Ratiometric measurements

Well scanning

6 to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)

Robot compatible

High energy xenon flash lamp

Two low-noise photomultiplier tubes
CCD spectrometer

Optical filters: ex and em wheels for 8 filters each
UV/vis absorbance spectrometer: full spectra or 8 distinct wavelengths in < 1 sec / well

Excitation and emission filter wheels for 8 filters each

Top: liquid filled light guides
Bottom: fiber optics

Filters
240 - 740 nm or 240 - 900 nm for FI, FP, TRF
240 - 740 nm for LUM
Spectrometer
220 - 1000 nm for ABS

FI
< 10 pM (< 0.2 fmol/well FITC, 384sv, 20 μL)
FP
< 5 mP SD at 1 nM FITC
TRF
< 30 amol/well europium
High-End TRF for Omega
< 3 amol/well europium
LUM
< 20 amol/well ATP
AlphaScreen^®
< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL)
ABS with spectrometer
Full spectrum captured in < 1 s/well
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD

Flying mode (1 flash)
9 s (96), 16 s (384)

Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing

Linear, orbital, and double-orbital with user-definable time and speed

System to inject an atmosphere or to pull a vacuum into the reader

+4 °C above ambient up to 45 °C or 65 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.

Multi-user Reader Control and MARS Data Analysis Software included.
FDA 21 CFR Part 11 compliant

Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg

Actively regulates O₂ and CO₂ - 0.1-20%

Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples.
Quality control internal standards (optional): four NIST-traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates.

Magazines for up to 50 plates - continuous loading feature

Microplate incubator and shaker

Excitation and emission filters

Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc.

LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates